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Keishiro Uda, Takeshi Yamagami, Sonoko Ishino, Christoph Gerle, Chai C.Gopalasingam, Hideki Shigematsu, Tomoyuki Numata, and Yoshizumi Ishino. Substrate specificity and action mechanism of the HerA-NurA nuclease from the hyperthermophilic archaeon Thermococcus kodakarensis. mBio, 2026 DOI: 10.1128/mbio.03523-25
Kazuki Ishihara, Shunsuke Matsumoto, Christoph Gerle, Chai C. Gopalasingam, Hideki Shigematsu, Tsuyoshi Shirai, Tomoyuki Numata (2026) Sequential structural rearrangements at the PAM-distal site of a type I-F3 CRISPR-Cas effector enabling RNA-guided DNA transposition. Nucleic Acids Res. doi: 10.1093/nar/gkaf1415
Kazuki Ishihara, Sumire Kitagawa, Naruhiko Adachi, Masato Akutsu, Toshiya Senda, Hideko Inanaga, Tomoyuki Numata (2025) Cryo-EM structure of Archaeoglobus fulgidus type III-B CRISPR-Cas effector and intermediate crRNA processing during effector assembly. Biochem. Biophys. Res. Commun. doi: 10.1016/j.bbrc.2025.152978
Matsumoto S, Kogure Y, Ono S, Numata T, Endo T. Msp1 and Pex19-Pex3 cooperate to achieve correct localization of Pex15 to peroxisomes. FEBS J. 2025 Aug;292(16):4289-4313. doi: 10.1111/febs.70132. Epub 2025 May 9. PMID: 40344504; PMCID: PMC12366275.
Parmar S, Bume DD, Connelly CM, Boer RE, Prestwood PR, Wang Z, Labuhn H, Sinnadurai K, Feri A, Ouellet J, Homan P, Numata T, Schneekloth JS Jr. Mechanistic analysis of Riboswitch Ligand interactions provides insights into pharmacological control over gene expression. Nat Commun. 2024 Sep 17;15(1):8173. doi: 10.1038/s41467-024-52235-3. PMID: 39289353; PMCID: PMC11408619.
Parmar S, Bume DD, Conelly C, Boer R, Prestwood PR, Wang Z, Labuhn H, Sinnadurai K, Feri A, Ouellet J, Homan P, Numata T, Schneekloth JS Jr. Mechanistic Analysis of Riboswitch Ligand Interactions Provides Insights into Pharmacological Control over Gene Expression. bioRxiv [Preprint]. 2024 Feb 23:2024.02.23.581746. doi: 10.1101/2024.02.23.581746. Update in: Nat Commun. 2024 Sep 17;15(1):8173. doi: 10.1038/s41467-024-52235-3. PMID: 38903087; PMCID: PMC11188086.
Tanaka J, Takashima T, Abe N, Fukamizo T, Numata T, Ohnuma T. Characterization of two rice GH18 chitinases belonging to family 8 of plant pathogenesis-related proteins. Plant Sci. 2023 Jan;326:111524. doi: 10.1016/j.plantsci.2022.111524. Epub 2022 Oct 31. PMID: 36328178.
Kitaoku Y, Taira T, Numata T, Ohnuma T, Fukamizo T. Structure, mechanism, and phylogeny of LysM-chitinase conjugates specifically found in fern plants. Plant Sci. 2022 Aug;321:111310. doi: 10.1016/j.plantsci.2022.111310. Epub 2022 May 6. PMID: 35696910.
Matsumoto S, Ono S, Shinoda S, Kakuta C, Okada S, Ito T, Numata T, Endo T. GET pathway mediates transfer of mislocalized tail-anchored proteins from mitochondria to the ER. J Cell Biol. 2022 Jun 6;221(6):e202104076. doi: 10.1083/jcb.202104076. Epub 2022 Apr 20. PMID: 35442388; PMCID: PMC9026240.
Kawamoto D, Takashima T, Fukamizo T, Numata T, Ohnuma T. A conserved loop structure of GH19 chitinases assists the enzyme function from behind the core-functional region. Glycobiology. 2022 Mar 31;32(4):356-364. doi: 10.1093/glycob/cwab117. PMID: 34939106.
Balaratnam S, Rhodes C, Bume DD, Connelly C, Lai CC, Kelley JA, Yazdani K, Homan PJ, Incarnato D, Numata T, Schneekloth JS Jr. A chemical probe based on the PreQ<sub>1</sub> metabolite enables transcriptome-wide mapping of binding sites. Nat Commun. 2021 Oct 6;12(1):5856. doi: 10.1038/s41467-021-25973-x. PMID: 34615874; PMCID: PMC8494917.
Oki K, Nagata M, Yamagami T, Numata T, Ishino S, Oyama T, Ishino Y. Family D DNA polymerase interacts with GINS to promote CMG-helicase in the archaeal replisome. Nucleic Acids Res. 2022 Apr 22;50(7):3601-3615. doi: 10.1093/nar/gkab799. PMID: 34568951; PMCID: PMC9023282.
Teramoto T, Koyasu T, Adachi N, Kawasaki M, Moriya T, Numata T, Senda T, Kakuta Y. Minimal protein-only RNase P structure reveals insights into tRNA precursor recognition and catalysis. J Biol Chem. 2021 Sep;297(3):101028. doi: 10.1016/j.jbc.2021.101028. Epub 2021 Jul 31. PMID: 34339732; PMCID: PMC8405995.
Oki K, Yamagami T, Nagata M, Mayanagi K, Shirai T, Adachi N, Numata T, Ishino S, Ishino Y. DNA polymerase D temporarily connects primase to the CMG-like helicase before interacting with proliferating cell nuclear antigen. Nucleic Acids Res. 2021 May 7;49(8):4599-4612. doi: 10.1093/nar/gkab243. PMID: 33849056; PMCID: PMC8096248.
Takashima T, Taku T, Yamanaka T, Fukamizo T, Numata T, Ohnuma T. Crystal structure and biochemical characterization of CJP38, a β-1,3-glucanase and allergen of Cryptomeria japonica pollen. Mol Immunol. 2019 Dec;116:199-207. doi: 10.1016/j.molimm.2019.10.016. Epub 2019 Nov 12. PMID: 31731097.
Connelly CM, Numata T, Boer RE, Moon MH, Sinniah RS, Barchi JJ, Ferré-D'Amaré AR, Schneekloth JS Jr. Synthetic ligands for PreQ<sub>1</sub> riboswitches provide structural and mechanistic insights into targeting RNA tertiary structure. Nat Commun. 2019 Apr 2;10(1):1501. doi: 10.1038/s41467-019-09493-3. PMID: 30940810; PMCID: PMC6445138.
Takeshita D, Sato M, Inanaga H, Numata T. Crystal Structures of Csm2 and Csm3 in the Type III-A CRISPR-Cas Effector Complex. J Mol Biol. 2019 Feb 15;431(4):748-763. doi: 10.1016/j.jmb.2019.01.009. Epub 2019 Jan 11. PMID: 30639408.
Takashima T, Numata T, Taira T, Fukamizo T, Ohnuma T. Structure and Enzymatic Properties of a Two-Domain Family GH19 Chitinase from Japanese Cedar (Cryptomeria japonica) Pollen. J Agric Food Chem. 2018 Jun 6;66(22):5699-5706. doi: 10.1021/acs.jafc.8b01140. Epub 2018 May 23. PMID: 29756783.
Asano K, Suzuki T, Saito A, Wei FY, Ikeuchi Y, Numata T, Tanaka R, Yamane Y, Yamamoto T, Goto T, Kishita Y, Murayama K, Ohtake A, Okazaki Y, Tomizawa K, Sakaguchi Y, Suzuki T. Metabolic and chemical regulation of tRNA modification associated with taurine deficiency and human disease. Nucleic Acids Res. 2018 Feb 28;46(4):1565-1583. doi: 10.1093/nar/gky068. PMID: 29390138; PMCID: PMC5829720.
Ohnuma T, Taira T, Umemoto N, Kitaoku Y, Sørlie M, Numata T, Fukamizo T. Crystal structure and thermodynamic dissection of chitin oligosaccharide binding to the LysM module of chitinase-A from Pteris ryukyuensis. Biochem Biophys Res Commun. 2017 Dec 16;494(3-4):736-741. doi: 10.1016/j.bbrc.2017.08.143. Epub 2017 Sep 1. PMID: 28867184.
Kitaoku Y, Fukamizo T, Numata T, Ohnuma T. Chitin oligosaccharide binding to the lysin motif of a novel type of chitinase from the multicellular green alga, Volvox carteri. Plant Mol Biol. 2017 Jan;93(1-2):97-108. doi: 10.1007/s11103-016-0549-5. Epub 2016 Nov 2. PMID: 27807643.
Shinya S, Nishimura S, Kitaoku Y, Numata T, Kimoto H, Kusaoke H, Ohnuma T, Fukamizo T. Mechanism of chitosan recognition by CBM32 carbohydrate-binding modules from a Paenibacillus sp. IK-5 chitosanase/glucanase. Biochem J. 2016 Apr 15;473(8):1085-95. doi: 10.1042/BCJ20160045. Epub 2016 Mar 2. PMID: 26936968.
Umemoto N, Ohnuma T, Osawa T, Numata T, Fukamizo T. Modulation of the transglycosylation activity of plant family GH18 chitinase by removing or introducing a tryptophan side chain. FEBS Lett. 2015 Aug 19;589(18):2327-33. doi: 10.1016/j.febslet.2015.07.018. Epub 2015 Jul 26. PMID: 26216755.
Osawa T, Inanaga H, Numata T. Crystallization and preliminary X-ray diffraction analysis of the CRISPR-Cas RNA-silencing Cmr complex. Acta Crystallogr F Struct Biol Commun. 2015 Jun;71(Pt 6):735-40. doi: 10.1107/S2053230X15007104. Epub 2015 May 22. PMID: 26057804; PMCID: PMC4461339.
Kitaoku Y, Umemoto N, Ohnuma T, Numata T, Taira T, Sakuda S, Fukamizo T. A class III chitinase without disulfide bonds from the fern, Pteris ryukyuensis: crystal structure and ligand-binding studies. Planta. 2015 Oct;242(4):895-907. doi: 10.1007/s00425-015-2330-4. Epub 2015 May 22. PMID: 25998529.
Osawa T, Inanaga H, Sato C, Numata T. Crystal structure of the CRISPR-Cas RNA silencing Cmr complex bound to a target analog. Mol Cell. 2015 May 7;58(3):418-30. doi: 10.1016/j.molcel.2015.03.018. Epub 2015 Apr 23. PMID: 25921071.
Umemoto N, Kanda Y, Ohnuma T, Osawa T, Numata T, Sakuda S, Taira T, Fukamizo T. Crystal structures and inhibitor binding properties of plant class V chitinases: the cycad enzyme exhibits unique structural and functional features. Plant J. 2015 Apr;82(1):54-66. doi: 10.1111/tpj.12785. Epub 2015 Mar 1. PMID: 25652217.
Suzuki T, Numata T. Convergent evolution of AUA decoding in bacteria and archaea. RNA Biol. 2014;11(12):1586-96. doi: 10.4161/15476286.2014.992281. PMID: 25629511; PMCID: PMC4615378.
Numata T, Inanaga H, Sato C, Osawa T. Crystal structure of the Csm3-Csm4 subcomplex in the type III-A CRISPR-Cas interference complex. J Mol Biol. 2015 Jan 30;427(2):259-73. doi: 10.1016/j.jmb.2014.09.029. Epub 2014 Oct 30. PMID: 25451598.
Numata T. Mechanisms of the tRNA wobble cytidine modification essential for AUA codon decoding in prokaryotes. Biosci Biotechnol Biochem. 2015;79(3):347-53. doi: 10.1080/09168451.2014.975185. Epub 2014 Oct 28. PMID: 25348586.
Ohnuma T, Umemoto N, Nagata T, Shinya S, Numata T, Taira T, Fukamizo T. Crystal structure of a "loopless" GH19 chitinase in complex with chitin tetrasaccharide spanning the catalytic center. Biochim Biophys Acta. 2014 Apr;1844(4):793-802. doi: 10.1016/j.bbapap.2014.02.013. Epub 2014 Feb 25. PMID: 24582745.
Ohnuma T, Umemoto N, Taira T, Fukamizo T, Numata T. Crystallization and preliminary X-ray diffraction analysis of an active-site mutant of `loopless' family GH19 chitinase from Bryum coronatum in a complex with chitotetraose. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Dec;69(Pt 12):1360-2. doi: 10.1107/S1744309113028935. Epub 2013 Nov 28. PMID: 24316830; PMCID: PMC3855720.
Ohnuma T, Umemoto N, Kondo K, Numata T, Fukamizo T. Complete subsite mapping of a "loopful" GH19 chitinase from rye seeds based on its crystal structure. FEBS Lett. 2013 Aug 19;587(16):2691-7. doi: 10.1016/j.febslet.2013.07.008. Epub 2013 Jul 18. PMID: 23871710.
Osawa T, Inanaga H, Numata T. Crystallization and preliminary X-ray diffraction analysis of the Cmr2-Cmr3 subcomplex in the CRISPR-Cas RNA-silencing effector complex. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 May 1;69(Pt 5):585-7. doi: 10.1107/S1744309113011202. Epub 2013 Apr 30. PMID: 23695584; PMCID: PMC3660908.
Osawa T, Inanaga H, Numata T. Crystal structure of the Cmr2-Cmr3 subcomplex in the CRISPR-Cas RNA silencing effector complex. J Mol Biol. 2013 Oct 23;425(20):3811-23. doi: 10.1016/j.jmb.2013.03.042. Epub 2013 Apr 10. PMID: 23583914.
Numata T. [Molecular basis for tRNA(Ile) agmatinylation essential for AUA codon decoding]. Seikagaku. 2012 Dec;84(12):1004-8. Japanese. PMID: 23350510.
Ogata M, Umemoto N, Ohnuma T, Numata T, Suzuki A, Usui T, Fukamizo T. A novel transition-state analogue for lysozyme, 4-O-β-tri-N-acetylchitotriosyl moranoline, provided evidence supporting the covalent glycosyl-enzyme intermediate. J Biol Chem. 2013 Mar 1;288(9):6072-82. doi: 10.1074/jbc.M112.439281. Epub 2013 Jan 9. PMID: 23303182; PMCID: PMC3585046.
Ohnuma T, Numata T, Osawa T, Inanaga H, Okazaki Y, Shinya S, Kondo K, Fukuda T, Fukamizo T. Crystal structure and chitin oligosaccharide-binding mode of a 'loopful' family GH19 chitinase from rye, Secale cereale, seeds. FEBS J. 2012 Oct;279(19):3639-3651. doi: 10.1111/j.1742-4658.2012.08723.x. Epub 2012 Sep 3. PMID: 22831795.
Umemoto N, Ohnuma T, Urpilainen H, Yamamoto T, Numata T, Fukamizo T. Role of tryptophan residues in a class V chitinase from Nicotiana tabacum. Biosci Biotechnol Biochem. 2012;76(4):778-84. doi: 10.1271/bbb.110914. Epub 2012 Apr 7. PMID: 22484953.
Osawa T, Inanaga H, Kimura S, Terasaka N, Suzuki T, Numata T. Crystallization and preliminary X-ray diffraction analysis of an archaeal tRNA-modification enzyme, TiaS, complexed with tRNA(Ile2) and ATP. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Nov 1;67(Pt 11):1414-6. doi: 10.1107/S1744309111034890. Epub 2011 Oct 27. PMID: 22102245; PMCID: PMC3212464.
Osawa T, Kimura S, Terasaka N, Inanaga H, Suzuki T, Numata T. Structural basis of tRNA agmatinylation essential for AUA codon decoding. Nat Struct Mol Biol. 2011 Oct 16;18(11):1275-80. doi: 10.1038/nsmb.2144. PMID: 22002223.
Terasaka N, Kimura S, Osawa T, Numata T, Suzuki T. Biogenesis of 2-agmatinylcytidine catalyzed by the dual protein and RNA kinase TiaS. Nat Struct Mol Biol. 2011 Oct 16;18(11):1268-74. doi: 10.1038/nsmb.2121. PMID: 22002222.
Ohnuma T, Numata T, Osawa T, Mizuhara M, Lampela O, Juffer AH, Skriver K, Fukamizo T. A class V chitinase from Arabidopsis thaliana: gene responses, enzymatic properties, and crystallographic analysis. Planta. 2011 Jul;234(1):123-37. doi: 10.1007/s00425-011-1390-3. Epub 2011 Mar 9. PMID: 21390509.
Toh Y, Takeshita D, Nagaike T, Numata T, Tomita K. Mechanism for the alteration of the substrate specificities of template-independent RNA polymerases. Structure. 2011 Feb 9;19(2):232-43. doi: 10.1016/j.str.2010.12.006. PMID: 21300291.
Ohnuma T, Numata T, Osawa T, Mizuhara M, Vårum KM, Fukamizo T. Crystal structure and mode of action of a class V chitinase from Nicotiana tabacum. Plant Mol Biol. 2011 Feb;75(3):291-304. doi: 10.1007/s11103-010-9727-z. Epub 2011 Jan 15. PMID: 21240541.
Ohnuma T, Osawa T, Fukamizo T, Numata T. Crystallization and preliminary X-ray diffraction analysis of a class V chitinase from Nicotiana tabacum. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Dec 1;66(Pt 12):1599-601. doi: 10.1107/S1744309110039060. Epub 2010 Nov 25. PMID: 21139204; PMCID: PMC2998363.
Ikeuchi Y, Kimura S, Numata T, Nakamura D, Yokogawa T, Ogata T, Wada T, Suzuki T, Suzuki T. Agmatine-conjugated cytidine in a tRNA anticodon is essential for AUA decoding in archaea. Nat Chem Biol. 2010 Apr;6(4):277-82. doi: 10.1038/nchembio.323. Epub 2010 Feb 7. PMID: 20139989.
Toh Y, Takeshita D, Numata T, Fukai S, Nureki O, Tomita K. Mechanism for the definition of elongation and termination by the class II CCA-adding enzyme. EMBO J. 2009 Nov 4;28(21):3353-65. doi: 10.1038/emboj.2009.260. Epub 2009 Sep 10. PMID: 19745807; PMCID: PMC2776095.
Osawa T, Ito K, Inanaga H, Nureki O, Tomita K, Numata T. Conserved cysteine residues of GidA are essential for biogenesis of 5-carboxymethylaminomethyluridine at tRNA anticodon. Structure. 2009 May 13;17(5):713-24. doi: 10.1016/j.str.2009.03.013. PMID: 19446527.
Osawa T, Inanaga H, Numata T. Crystallization and preliminary X-ray diffraction analysis of the tRNA-modification enzyme GidA from Aquifex aeolicus. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 May 1;65(Pt 5):508-11. doi: 10.1107/S1744309109013591. Epub 2009 Apr 24. PMID: 19407389; PMCID: PMC2675597.
Toh Y, Numata T, Watanabe K, Takeshita D, Nureki O, Tomita K. Molecular basis for maintenance of fidelity during the CCA-adding reaction by a CCA-adding enzyme. EMBO J. 2008 Jul 23;27(14):1944-52. doi: 10.1038/emboj.2008.124. Epub 2008 Jun 26. PMID: 18583961; PMCID: PMC2486279.
Tomita K, Numata T, Fukai T, Nureki S, Ishitani R, Nureki O. Animated crystallography of genetic code translation. Nucleic Acids Symp Ser (Oxf). 2007;(51):101-2. doi: 10.1093/nass/nrm051. PMID: 18029606.
Numata T, Ikeuchi Y, Fukai S, Suzuki T, Nureki O. Snapshots of tRNA sulphuration via an adenylated intermediate. Nature. 2006 Jul 27;442(7101):419-24. doi: 10.1038/nature04896. PMID: 16871210.
Oshikane H, Sheppard K, Fukai S, Nakamura Y, Ishitani R, Numata T, Sherrer RL, Feng L, Schmitt E, Panvert M, Blanquet S, Mechulam Y, Söll D, Nureki O. Structural basis of RNA-dependent recruitment of glutamine to the genetic code. Science. 2006 Jun 30;312(5782):1950-4. doi: 10.1126/science.1128470. PMID: 16809540.
Tsukazaki T, Mori H, Fukai S, Numata T, Perederina A, Adachi H, Matsumura H, Takano K, Murakami S, Inoue T, Mori Y, Sasaki T, Vassylyev DG, Nureki O, Ito K. Purification, crystallization and preliminary X-ray diffraction of SecDF, a translocon-associated membrane protein, from Thermus thermophilus. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Apr 1;62(Pt 4):376-80. doi: 10.1107/S1744309106007779. Epub 2006 Mar 25. PMID: 16582489; PMCID: PMC2222563.
Numata T, Ikeuchi Y, Fukai S, Adachi H, Matsumura H, Takano K, Murakami S, Inoue T, Mori Y, Sasaki T, Suzuki T, Nureki O. Crystallization and preliminary X-ray analysis of the tRNA thiolation enzyme MnmA from Escherichia coli complexed with tRNA(Glu). Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Apr 1;62(Pt 4):368-71. doi: 10.1107/S174430910600738X. Epub 2006 Mar 10. PMID: 16582487; PMCID: PMC2222564.
Fukuhara H, Kifusa M, Watanabe M, Terada A, Honda T, Numata T, Kakuta Y, Kimura M. A fifth protein subunit Ph1496p elevates the optimum temperature for the ribonuclease P activity from Pyrococcus horikoshii OT3. Biochem Biophys Res Commun. 2006 May 12;343(3):956-64. doi: 10.1016/j.bbrc.2006.02.192. Epub 2006 Mar 15. PMID: 16574071.
Kakuta Y, Ishimatsu I, Numata T, Kimura K, Yao M, Tanaka I, Kimura M. Crystal structure of a ribonuclease P protein Ph1601p from Pyrococcus horikoshii OT3: an archaeal homologue of human nuclear ribonuclease P protein Rpp21. Biochemistry. 2005 Sep 13;44(36):12086-93. doi: 10.1021/bi050738z. PMID: 16142906.
Kimura K, Numata T, Kakuta Y, Kimura M. Amino acids conserved at the C-terminal half of the ribonuclease T2 family contribute to protein stability of the enzymes. Biosci Biotechnol Biochem. 2004 Aug;68(8):1748-57. doi: 10.1271/bbb.68.1748. PMID: 15322360.
Numata T, Ishimatsu I, Kakuta Y, Tanaka I, Kimura M. Crystal structure of archaeal ribonuclease P protein Ph1771p from Pyrococcus horikoshii OT3: an archaeal homolog of eukaryotic ribonuclease P protein Rpp29. RNA. 2004 Sep;10(9):1423-32. doi: 10.1261/rna.7560904. PMID: 15317976; PMCID: PMC1370628.
Takagi H, Watanabe M, Kakuta Y, Kamachi R, Numata T, Tanaka I, Kimura M. Crystal structure of the ribonuclease P protein Ph1877p from hyperthermophilic archaeon Pyrococcus horikoshii OT3. Biochem Biophys Res Commun. 2004 Jul 2;319(3):787-94. doi: 10.1016/j.bbrc.2004.05.055. PMID: 15184052.
Kouzuma Y, Mizoguchi M, Takagi H, Fukuhara H, Tsukamoto M, Numata T, Kimura M. Reconstitution of archaeal ribonuclease P from RNA and four protein components. Biochem Biophys Res Commun. 2003 Jul 4;306(3):666-73. doi: 10.1016/s0006-291x(03)01034-9. PMID: 12810070.
Numata T, Suzuki A, Kakuta Y, Kimura K, Yao M, Tanaka I, Yoshida Y, Ueda T, Kimura M. Crystal structures of the ribonuclease MC1 mutants N71T and N71S in complex with 5'-GMP: structural basis for alterations in substrate specificity. Biochemistry. 2003 May 13;42(18):5270-8. doi: 10.1021/bi034103g. PMID: 12731868.
Numata, T. and Kimura, M. Contribution of Gln9 and Phe80 to substrate binding in ribonuclease MC1 from bitter gourd seeds. J. Biochem. 130, 621-626 (2001).
Numata, T., Suzuki, A., Yao, M., Tanaka, I. and *Kimura, M. Amino acid residues in ribonuclease MC1 from bitter gourd seeds which are essential for uridine specificity. Biochemistry 40, 524-530 (2001).
Suzuki, A., Yao, M., Tanaka, I., Numata, T., Kikukawa, S., Yamasaki, N. and *Kimura, M. Crystal structures of the ribonuclease MC1 from bitter gourd seeds, complexed with 2’-UMP or 3’-UMP, reveal structural basis for uridine specificity. Biochem. Biophys. Res. Commun. 275, 572-576 (2000).
Numata, T., Kashiba, T., Hino, M., Funatsu, G., Ishiguro, M., Yamasaki, N. and *Kimura, M. Expression and mutational analysis of amino acid residues involved in catalytic activity in a ribonuclease MC1 from the seeds of bitter gourd. Biosci. Biotechnol. Biochem. 64, 603-605 (2000).
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